한빛사 논문
Nayab Batool1,2, Seokyoung Yoon3, Saba Imdad1, Minsuk Kong4, Hun Kim1, Sangryeol Ryu5, Jung Heon Lee3*, Akhilesh Kumar Chaurasia1*, and Kyeong Kyu Kim1*
1Department of Precision Medicine, Institute for Antimicrobial Resistance Research and Therapeutics, Graduate School of Basic Medical Sciences (GSBMS), Sungkyunkwan University School of Medicine, Suwon 16419, Korea
2Institute of Microbiology, University of Agriculture, Faisalabad (UAF) 38000, Pakistan.
3Biological & Nanoscale Materials Lab, School of Advanced Materials Science & Engineering, Sungkyunkwan University (SKKU), Suwon 16419, Korea.
4Department of Food Science and Technology, Seoul National University od Science and Technology, Seoul 01811, Korea.
5Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute of Agriculture and Life Sciences, Seoul National University (SNU), Seoul 08826, Korea.
CORRESPONDING AUTHORS
Jung H. Lee, Akhilesh K. Chaurasia, Kyeong K. Kim
Abstract
Methicillin‐resistant Staphylococcus aureus (MRSA) causes diseases ranging from skin infections to lethal sepsis and has become a serious threat to human health due to multiple‐drug resistance (MDR). Therefore, a resistance‐free antibacterial therapy is necessary to overcome MDR MRSA infections. In this study, an antibacterial nanorobot (Ab‐nanobot) is developed wherein a cell wall‐binding domain (CBD)‐endolysin, acting as a sensor, is covalently conjugated with an actuator consisting of an iron oxide/silica core–shell. The CBD‐endolysin sensor shows an excellent specificity to detect, bind, and accumulate on the S. aureus USA300 cell surface even in a bacterial consortium, and in host cell infections. Ab‐nanobot specifically captures and kills MRSA in response to medically approved radiofrequency (RF) electromagnetic stimulation (EMS) signal. When Ab‐nanobot receives the RF‐EMS signal on the cell surface, actuator induces cell death in MRSA with 99.999% removal within 20 min by cell‐wall damage via generation of localized heat and reactive oxygen species. The in vivo efficacy of Ab‐nanobot is proven using a mice subcutaneous skin infection model. Collectively, this study offers a nanomedical resistance‐free strategy to overcome MDR MRSA infections by providing a highly specific nanorobot for S. aureus.
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