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HPLC/MS 분석결과...
레벨1 무상인생 (과기인)
분석시료를 hplc/ms 로 분석시켜 봤는데 이런 결과가나오네요.. 처음보는 그래프인데 단순히 오염일까요..
메소드/조건 기입해봅니다..
Parameter Condition
LC/MS Model AB Sciex TripleTOF 5600 System Model 1034600R/L, IDEX
calibrant delivery system
HPLC System Agilent 1260 Binary Pump, Agilent 1260 Degasser,
Agilent 1290 Column Oven, Agilent Instant Pilot G4208A, CTC
Autosampler HTC-xt
Column Waters CORTECS C18, 150 x 2.1 mm, 2.7 µm
Column Guard Phenomenex C18 SecurityGuard 4 x 2.0 mm
Column Temperature 45 °C
Injection Volume 3 µL
Flow Rate 0.350 mL/min
Scan Range 80 to 1500 m/z
Ion source gas 1 (GS 1) 40 psi
Ion source gas 2 (GS 2) 60 psi
Curtain gas (CUR) 35 psi
Dry Gas temperature (TEM) 400 °C
Ion spray voltage (IS) 4500 V (4.5 kV)
Mobile Phase A 5 mM ammonium acetate in water
Mobile Phase B 50:50 (v/v) methanol:acetonitrile
Gradient 15% B at 0 minutes to 15% B at 1.20 minutes to 100% B at
18 minutes, hold to 30 minutes, back to 15% B at 32 minutes
and hold to 15% B until 36 minutes
Ionization ESI positive, ESI negative
Library Eurofins Substance Library
NIST 2017 MS/MS Database converted and condensed for
use with SCIEX software. Version 1.0
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