한빛사 논문
Abstract
Nohyun Leea,1, Hyoungsu Kimb,1, Seung Hong Choib, Mihyun Parka, Dokyoon Kima, Hyo-Cheol Kimb, Yoonseok Choib, Shunmei Linb, Byung Hyo Kima, Hye Seung Jungc Hyeonjin Kimb,d, Kyong Soo Parkc, Woo Kyung Moonb,2, and Taeghwan Hyeona,2
aNational Creative Research Initiative Center for Oxide Nanocrystalline Materials, World Class University Program of Chemical Convergence for Energy and Environment, and School of Chemical and Biological Engineering, Seoul National University, Seoul 151-744, Korea;
bDiagnostic Radiology, Seoul National University Hospital, and the Institute of Radiation Medicine, Medical Research Center, Seoul National University, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea;
cDepartment of Internal Medicine, Seoul National University College of Medicine, 28 Yeongeon-dong, Jongno-gu, Seoul 110-744, Korea; and
dLee Gil Ya Cancer and Diabetes Institute, Gachon University of Medicine and Science, Incheon 406-840, Korea
Edited by Nicholas J. Turro, Columbia University, New York, NY 10027, and approved January 3, 2011 (received for review November 5, 2010)
1N.L. and Hyoungsu Kim contributed equally to this work.
Abstract
For ultrasensitive magnetic resonance imaging (MRI), magnetic nanoparticles with extremely high r2 relaxivity are strongly desired. Magnetosome-like nanoparticles were prepared by coating polyethylene glycol-phospholipid (PEG-phospholipid) onto ferrimagnetic iron oxide nanocubes (FIONs). FIONs exhibited a very high relaxivity (r2) of 324 mM-1 s-1, allowing efficient labeling of various kinds of cells. The magnetic resonance (MR) imaging of single cells labeled with FIONs is demonstrated not only in vitro but also in vivo. Pancreatic islet grafts and their rejection could be imaged using FIONs on a 1.5 T clinical MRI scanner. The strong contrast effect of FIONs enabled MR imaging of transplanted islets in small rodents as well as in large animals. Therefore, we expect that MR imaging of pancreatic islet grafts using FIONs has the potentials for clinical applications. Furthermore, FIONs will enable highly sensitive noninvasive assessment after cell transplantation.
cell tracking, contrast agent, molecular imaging, diabetes, islet transplantation
Footnotes
2To whom correspondence may be addressed.
Author contributions: N.L., Hyoungsu Kim, W.K.M., and T.H. designed research; N.L., Hyoungsu Kim, S.H.C., M.P., D.K., H.-C.K., Y.S.C., S.L., and B.H.K. performed research; N.L., S.H.C., H.S.J., Hyeonjin Kim, and K.S.P. analyzed data; and N.L., Hyoungsu Kim, W.K.M., and T.H. wrote the paper.
The authors declare no conflict of interest.
This article is a PNAS Direct Submission.
This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1016409/-/DCSupplemental.
Freely available online through the PNAS open access option.
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