한빛사 논문
Abstract
Bongseo Choi†§, Hyojin Moon†§, Sung Joon Hong†, Changsik Shin†, Yoonkyung Do†, Seongho Ryu*‡, and Sebyung Kang*†
† Department of Biological Sciences, School of Life Sciences, Ulsan National Institute of Science and Technology (UNIST), Ulsan, 689-798, Korea
‡ Soonchunhyang Institute of Medi-bio Science (SIMS), Soonchunhyang University, Cheonan, 336-745, Korea
§ B. Choi and H. Moon contributed equally.
*Corresponding Authors
Abstract
In cancer immunotherapy, robust and efficient activation of cytotoxic CD8+ T cell immune responses is a promising, but challenging task. Dendritic cells (DCs) are well-known professional antigen presenting cells that initiate and regulate antigen-specific cytotoxic CD8+ T cells that kill their target cells directly as well as secrete IFN-γ, a cytokine critical in tumor rejection. Here, we employed recently established protein cage nanoparticles, encapsulin (Encap), as antigenic peptide nanocarriers by genetically incorporating the OT-1 peptide of ovalbumin (OVA) protein to the three different positions of the Encap subunit. With them, we evaluated their efficacy in activating DC-mediated antigen-specific T cell cytotoxicity and consequent melanoma tumor rejection in vivo. DCs efficiently engulfed Encap and its variants (OT-1-Encaps), which carry antigenic peptides at different positions, and properly processed them within phagosomes. Delivered OT-1 peptides were effectively presented by DCs to naive CD8+ T cells successfully, resulting in the proliferation of antigen-specific cytotoxic CD8+ T cells. OT-1-Encap vaccinations in B16-OVA melanoma tumor bearing mice effectively activated OT-1 peptide specific cytotoxic CD8+ T cells before or even after tumor generation, resulting in significant suppression of tumor growth in prophylactic as well as therapeutic treatments. A large number of cytotoxic CD8+ T cells that actively produce both intracellular and secretory IFN-γ were observed in tumor-infiltrating lymphocytes collected from B16-OVA tumor masses originally vaccinated with OT-1-Encap-C upon tumor challenges. The approaches we describe herein may provide opportunities to develop epitope-dependent vaccination systems that stimulate and/or modulate efficient and epitope-specific cytotoxic T cell immune responses in nonpathogenic diseases.
Keywords: antigen delivery; cytotoxic T cells; dendritic cells; encapsulin; vaccination
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