한빛사 논문
POSTECH
Abstract
Hwan Myung Kim1, Hyo-Jung Choo2, Soon-Young Jung2, Young-Gyu Ko Prof. Dr.2,*, Won-Hwa Park1, Seung-Joon Jeon Prof. Dr.1,*, Chul Hoon Kim3, Taiha Joo Prof. Dr.3,* and Bong Rae Cho Prof. Dr.1,*
1 Department of Chemistry and Center for Electro- and Photo-Responsive Molecules, Korea University, 1-Anamdong, Seoul, 136-701, Korea
2 Graduate School of Life Sciences and Biotechnology, Korea University, 1-Anamdong, Seoul, 136-701, Korea
3 Department of Chemistry, Pohang University of Science and Technology, Pohang 790784, Korea
*Corresponding authors
Abstract
The lipid-rafts hypothesis proposes that naturally occurring lipid aggregates exist in the plane of membrane that are involved in signal transduction, protein sorting, and membrane transport. To understand their roles in cell biology, a direct visualization of such domains in living cells is essential. For this purpose, 6-dodecanoyl-2-(dimethylamino)naphthalene (laurdan), a membrane probe that is sensitive to the polarity of the membrane, has often been used. We have synthesized and characterized 6-dodecanoyl-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (C-laurdan), which has the advantages of greater sensitivity to the membrane polarity, a brighter two-photon fluorescence image, and reflecting the cell environment more accurately than laurdan. Lipid rafts can be visualized by two-photon microscopy by using C-laurdan as a probe. Our results show that the lipid rafts cover 38% of the cell surface.
Keywords: C-laurdan; fluorescent probes; lipid rafts; membranes; two-photon microscopy; vesicles
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