한빛사 논문
한국기초과학지원연구원, 제주대학교
Abstract
Eun-Yi Koa,b,1 Su-Hyeon Choa,b,1, Seung Hae Kwona, Chi-Yong Eoma, Myeong Seon Jeonga, WonWoo Leeb, Seo-Young Kimb, Soo-Jin Heoc, Ginnae Ahnd, Kang Pa Leee, You-Jin Jeonb,* Kil-Nam Kima,f,*
aChuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of Korea
bDepartment of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea
c Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science and Technology, Jeju 63349, Republic of Korea
dDepartment of Marine Bio-food Science, College of Fisheries and Ocean Sciences, Chonnam National University, 59626, Republic of Korea
e Department of Physiology, School of Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea
f Department of Marin Biotechnology, University of Science and Technology, Daejeon 34113, Republic of Korea
1These authors contributed equally to this study.
* Corresponding authors
Abstract
In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. IκB was suppressed by PDTC, but not significantly. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.
Keywords: Zebrafish embryosInflammationPro-inflammatory mediatorROSNF-κB
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