Chang-Ho Kanga, YuJin Shina, HongSik Yub, SuKyung Kimb, Jae-Seong Soa,*
aDepartment of Biological Engineering, Inha University, Incheon, Republic of Korea
bWest Sea Fisheries Institute, National Fisheries Research & Development Institute (NFRDI), Incheon, Republic of Korea
*Corresponding author
Abstract
Vibrio parahaemolyticus, found frequently in oysters and other seafoods, is the most prevalent gastroenteritis-causing pathogen in Korea and other Asian countries. It is associated exclusively with the consumption of raw or improperly cooked contaminated seafood, especially oysters. In this study, we isolated and characterized 59 V. parahaemolyticus strains (toxR-positive) from May to October 2016 in shellfish-harvesting areas off the west coast of Korea. The results revealed that none of the isolates contained the tdh and trh toxicity genes. The multiple antibiotic resistance (MAR) value of most isolates was 0.32, but it was as high as 0.69 in one isolate strain. Moreover, when resistance to heavy metals was examined, the majority of the isolates displayed resistance to Ba2+ (98.3%), Co3+ (28.8%), Cd2+ (16.9%), and Cu2+ (13.6%). Interestingly our data revealed that tolerance to heavy metals was prevalent in the V. parahaemolyticus strains with more than two antibiotic resistance phenotypes.
Keywords : Vibrio parahaemolyticus, Oyster, Antibiotic, Heavy metal, Virulence genes