한빛사 논문
Abstract
Jeonghoon Hana,1, Jun Chul Parka,1, Hye-Min Kanga, Eunjin Byeona, Deok-Seo Yoona, Min-Chul Leea, Alaa El-Din H. Sayedb, Un-Ki Hwangc, Jae-Seong Leea,*
a Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea
b Department of Zoology, Faculty of Science, Assiut University, 71516 Assiut, Egypt
c Marine Ecological Risk Assessment Center, West Sea Fisheries Research Institute, National Institute of Fisheries Science, Incheon 46083, South Korea
*Corresponding author : Jae-Seong Lee
1These authors are equally contributed to this work.
Abstract
To examine the adverse effects of the benzo[α]pyrene (B[α]P), the monogonont rotifer Brachionus rotundiformis was exposed to various concentration of B[α]P (0 [control], 1, 10, and 100 μg/L) and measured life cycle parameters (e.g., mortality, fecundity [cumulated number of offspring], and lifespan), reactive oxygen species (ROS), antioxidant enzymatic activity of glutathione S-transferase (GST). In addition, defense-related transcripts (e.g., glutathione S-transferases [GSTs], ATP binding cassette [ABCs] transporters) and Western blot analysis of mitogen-activated protein kinase (MAPK) signaling pathway were investigated in B[α]P-exposed rotifer. In this study, the total intracellular ROS level and GST activity were significantly increased (P < 0.05), while fecundity and lifespan were also significantly (P < 0.05) reduced in a concentration dependent manner in B[α]P-exposed B. rotundiformis. In addition, transcriptional regulation of GSTs and ABC transporters were significantly upregulated and downregulated (P < 0.05), respectively, suggesting that B[α]P can induce oxidative stress leading to induction of antioxidant system and detoxification mechanism. In addition to detoxification-related genes, B[α]P-exposed B. rotundiformis showed the increased levels of the p-JNK and p-p38, suggesting that B[α]P can activate MAPK signaling pathway in B. rotundiformis.
Keywords : Polycyclic aromatic hydrocarbon; Detoxification; GSTs; ABC transporters
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