한빛사 논문
Ge Gao,1,a) Hyeok Kim,2,3,a) Byoung Soo Kim,1 Jeong Sik Kong,4 Jae Yeon Lee,1 Bong Woo Park,2,3 Suhun Chae,1 Jisoo Kim,4 Kiwon Ban,5 Jinah Jang,6 Hun-Jun Park,2,3,7,b) and Dong-Woo Cho1,b)
1 Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea
2 Department of Medical Life Science, College of Medicine, The Catholic University of Korea, Seoul 06591, South Korea
3 Division of Cardiology, Department of Internal Medicine, Seoul St. Mary’s Hospital, The Catholic University of Korea, Seoul 06591, South Korea
4 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea
5 Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong 999077, Hong Kong
6 Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, South Korea
7 Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, South Korea
a) Contributions: G. Gao and H. Kim contributed equally to this work.
b) Correspondence : Dong-Woo Cho, Dong-Woo Cho
Abstract
Tissue engineering has emerged as a promising approach to viable small-diameter vascular grafts that may be used to treat cardiovascular diseases. One challenge in constructing such blood vessels is proper localization of endothelial cells and smooth muscle cells, as well as promotion of their cellular functions to generate functional tissues. Thus far, construction of small-diameter vascular substitutes with both endothelial and muscular tissues, which is essential for the grafts to acquire antithrombosis function and sufficient strength to avoid thrombus formation as well as to withstand blood pressure, has not yet been demonstrated. In this study, we engineer small-diameter blood vessel grafts containing both functional endothelial and muscular cell layers, which has been demonstrated in vivo in a living rat model. Our construction of the blood vessel grafts uses vascular-tissue-derived extracellular matrix bioinks and a reservoir-assisted triple-coaxial cell printing technique. The prematured vessel was implanted for three weeks as a graft of rat abdominal aorta in a proof-of-concept study where all implants showed great patency, intact endothelium, remodeled smooth muscle, and integration with host tissues at the end of the study. These outcomes suggest that our approach to tissue-engineered biomimetic blood vessels provides a promising route for the construction of durable small-diameter vascular grafts that may be used in future treatments of cardiovascular diseases.
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