한빛사 논문
Mike Filius*,1, Tao Ju Cui*,1, Adithya Ananth1,2, Margreet Docter1, Jorrit W. Hegge3, John van der Oost3, Chirlmin Joo#,1
1Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, van der Maasweg 9, 2629HZ Delft, The Netherlands.
2Netherlands Organisation for Applied Scientific Research (TNO), P.O. Box 155, 2600 AD Delft, Netherlands.
3Laboratory of Microbiology, Wageningen University, Dreijenplein 10, 6703 HB Wageningen, The Netherlands.
*These authors contributed equally to this work
#Corresponding author
Abstract
Super-resolution imaging allows for visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA Point Accumulation In Nanoscale Topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of the imager strands. Here we report on a method where imager strands are loaded into a protein, Argonaute (Ago), that allows for faster binding. Ago pre-orders the DNA imager strand into a helical conformation, allowing for 10 times faster target binding. Using a 2D DNA origami structure, we demonstrate that Ago-assisted DNA-PAINT (Ago-PAINT) can speed up the current DNA-PAINT technique by an order of magnitude while maintaining the high spatial resolution. We envision this tool to be useful for super-resolution imaging and other techniques that rely on nucleic-acid interactions.
논문정보
관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기