한빛사 논문
건국대학교
Abstract
Ji-Eun Raa, So-Yeun Woob, Kwang-Sik Leeb, Mi Ja Leeb, Hyun Young Kimb, Hyeon Mi Hamb, Ill-Min Chunga, Du Hyun Kimc, Jin Hwan Leec,*, Woo Duck Seob,*
aDepartment of Crop Science, College of Sanghuh Life Science, Konkuk University, Seoul 05029, Republic of Korea
bDivision of Crop Foundation, National Institute of Crop Science, Rural Development Administration, Jellabuk-do 55365, Republic of Korea
cDepartment of Life Resources Industry, Dong-A University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, Republic of Korea
*Corresponding author
Abstract
Policosanols is a health promoting aliphatic alcohol known as lipid-lowing agent.
To enable maximising the functional properties of wheat, this research investigates the policosanol profiles and adenosine 5′-monophosphate-activated protein kinase (AMPK) activation potential of Korean wheat seedlings according to cultivars and growth times. GC-MS revealed six policosanols that differed markedly in content between 17 cultivars, especially, octacosanol (8) showed the most predominant component (49–83%), varying significantly in average concentrations with growth times as 361.4 (3 days) → 613.0 (6 days) → 203.1 (9 days) → 196.5 (12 days) → 50.9 mg/100 g (19 days). The highest average policosanol (738.7 mg/100 g) exhibited after 6 days, while the lowest was 104.4 mg/100 g on 19 days. Moreover, the wheat cultivars including Shinmichal 1, Anbaek, Namhae, and Joah at 6 days may be recommended as potential sources because of high policosanols (921.7–990.6 mg/100 g). Western blot analysis revealed markedly higher AMPK activation in cells treated with the hexane extracts (150–370% at 100 μg/ml) and octacosanol (8) possessed potent AMPK activator (control; 100 → 280% at 200 μg/ml). It is confirmed that the AMPK activation by wheat seedlings are positively related to the highest policosanol content at the 6 days of growth time, independent of the cultivar. Our results may be contributed to enhance the wheat value regarding development of new cultivars and functional foods.
Keywords:
Wheat seedling; Policosanol; Octacosanol; AMPK; Growth times; GC–MS
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