한빛사 논문
Abstract
Bhagwan S.Batule1, Youngung Seok1, Min-Gon Kim*
Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Gwangju 500-712, Republic of Korea
1Bhagwan Sahebrao Batule and Youngung Seok contributed equally.
*Corresponding author
Astract
Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (∼5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork (Sus scrofa), chicken (Gallus gallus), and beef (Bos taurus), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.
Keywords
paper chip; DNA extraction; mitochondrial DNA; PCR analysis; Meat detection
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