한빛사 논문
고려대학교
Chandra Khatua,†,⊥ Sunhong Min,†,⊥ Hee Joon Jung,§,∥,#,⊥ Jeong Eun Shin,†,⊥ Na Li,¶ Indong Jun,¶ Hui-Wen Liu,Δ Gunhyu Bae,† Hyojun Choi,† Min Jun Ko,† Yoo Sang Jeon,† Yu Jin Kim,□ Joonbum Lee,† Minji Ko,† Gyubo Shim,† Hongchul Shin,◊ Sangbum Lee,† Seok Chung,‡,Δ,∫ Young Keun Kim,†,‡ Jae-Jun Song,¶ Vinayak P. Dravid,§,∥,# and Heemin Kang†,‡,*
†Department of Materials Science and Engineering, Korea University, Seoul, Republic of Korea.
‡Department of Biomicrosystem Technology, Korea University, Seoul, Republic of Korea.
§Department of Materials Science and Engineering, Northwestern University, Evanston, IL, USA.
∥International Institute for Nanotechnology, Evanston, IL, USA.
#NUANCE Center, Northwestern University, Evanston, IL, USA.
¶Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.
ΔSchool of Mechanical Engineering, Korea University, Seoul, Republic of Korea.
□Institute for High Technology Materials and Devices, Korea University, Seoul, Republic of Korea.
∫KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, Republic of Korea. ◊Department of Biotechnology, Korea University, Seoul, Republic of Korea.
⊥C.K., S.M., H.J.J., and J.E.S. contributed equally to this work.
*Corresponding author
Abstract
Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.
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