한빛사 논문
전남대학교
Abstract
Rahul Krishnana, Jong-Oh Kimb, Syed Shariq Nazir Qadiria, Jae-Ok Kimc, Myung-Joo Oha,*
aDepartment of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea
bInstitute of Marine Biotechnology, Pukyong National University, Busan, Republic of Korea
cNational Institute of Fisheries Science, Busan, 46083, Republic of Korea
*Corresponding author
Abstract
In the present study, the early uptake of nervous necrosis virus (NNV) in the tissues (gill, brain, skin, eye, heart) and immune response associated with the uptake in the gill and brain of seven-band grouper was investigated. The gill was found to act as a primary portal of entry for NNV during the initial phase of the water-borne infection. The presence of viral genome and infectious particles was demonstrated using quantitative (qPCR, viral titer) and qualitative (ISH) approach. Initially, an increased viral uptake was noticed, but the virus got cleared from the gills at the later phase of infection. Localization in the brain was evident at the blood-brain barrier followed to the brain parenchyma in the latter stage of infection. Nectin-4, an established NNV receptor, and GHSC70 showed an up-regulated expression throughout the challenge period initially in the gill and latter phase in brain; however, it seems that the virus does not use gill as a primary replication site but brain as a permissive tissue. Combined activity as reflected by the up-regulation of cytokine, interferon, antigen-presenting cell, and immunoglobulin genes restricts early NNV replication in gill. Observations from the present study provide a better understanding of early NNV entry and also opens a window for further elucidating the modes of NNV neuro-invasion through systemic circulation.
Keywords
Nervous necrosis virus; Seven-band grouper; Early uptake; Immune response; In-situ hybridization
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