한빛사 논문
Intan Rosalina Suhito, Novi Angeline, Kwang-Ho Lee, Huijung Kim, Chun Gwon Park, Zhengtang Luo, and Tae-Hyung Kim*
I. R. Suhito, N. Angeline, K.-H. Lee, H. Kim, Prof. T.-H. Kim
School of Integrative Engineering
Chung-Ang University Seoul
06974, Republic of Korea
Prof. C. G. Park
Department of Biomedical Engineering
SKKU Institute for Convergence
Sungkyunkwan University (SKKU)
Suwon 16419, Republic of Korea
Prof. Z. Luo
Department of Chemical and Biological Engineering
Hong Kong University of Science and Technology
Kowloon, Hong Kong 999077, China
Prof. T.-H. Kim
Integrative Research Center for Two-Dimensional Functional Materials
Institute of Interdisciplinary Convergence Research
Chung-Ang University
Seoul 06974, Republic of Korea
*Corresponding author
Abstract
In this study, a multifunctional platform that enables the highly efficient formation of 3D multicellular cancer spheroids and precise real‐time assessments of the anticancer effects of curcumin in a brain tumor coculture model is reported. A highly conductive gold nanostructure (HCGN) is fabricated to facilitate cancer spheroid formation without using anti‐cell adhesion molecules. A neuroblastoma (SH‐SY5Y) and glioblastoma (U‐87MG) coculture model is generated on HCGN with a specific cell‐to‐cell ratio (SH‐SY5Y: U‐87MG = 1:1), and their redox behaviors are successfully measured without destroying the distinct 3D structure of the multicellular spheroids. Using electrochemical signals as an indicator of spheroid viability, the effects of potential anticancer compounds on cocultured spheroids are further assessed. Remarkably, decreased cell viability in 3D spheroids caused by a low concentration of curcumin (30 µM) is detectable using the electrochemical method (29.4%) but not with a conventional colorimetric assay (CCK‐8). The detection is repeated more than ten times for both short‐ (63 h) and long‐term cultivation (144 h) without damaging the spheroids, enabling real‐time, non‐destructive pharmacokinetic analysis of various drug candidates. Therefore, it can be concluded that the hybrid platform is a highly promising, precise, and high‐throughput drug screening tool based on 3D cell cultivation.
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