한빛사 논문
경상대학교
Dong-In Junga, Jeongim Had, Byeong-Teck Kangb, Ju-Won Kimb, Fu-Shi Quanf, Jong-Hwan Leec, Eung-Je Wooe, Hee-Myung Parkb,*
aDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 660-701, South Korea
bDepartment of Veterinary Internal Medicine, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea
cDepartment of Veterinary Anatomy, College of Veterinary Medicine, Konkuk University, Seoul 143-701, South Korea
dDepartment of Cell and Developmental Biology, School of Dentistry, DRI and Brain Korea 21 Program, Seoul National University, Seoul 110-749, South Korea
eCollege of Electronics and Information, Kyunghee University, Gyeonggi-do 446-701, South Korea
fDepartment of Anatomy, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea
*Corresponding author
Abstract
The purpose of this study is to compare the therapeutic effects between autologous and allogenic bone-marrow-derived mesenchymal stem cell (MSC) transplantation in experimentally-induced spinal cord injury (SCI) of dogs.
Thirty adult Beagle dogs (control group = 10, autologous group = 10, and allogenic group = 10) were used in this study. Prelabeled MSCs were intrathecally transplanted through the lumbar spinal cord into the injured lesion at a density of 1 × 107 cells 7 days after SCI. Neurological signs of dogs in both autologous and allogenic groups were improved in their pelvic limbs after SCI compared with those in control group. Both autologous and allogenic groups showed significantly higher the Olby scores than control group (p < 0.05). This finding was consistent with results of MRI and histopathological examination in both groups. Immunofluorescence analysis revealed that prelabeled autologous and allogenic MSCs were detected in the injured lesions both at 1 and 4 weeks after transplantation. However, the distribution ratio of MSCs on the injured lesion in allogenic group was significantly decreased at 4 weeks after transplantation relatively to at 1 week after transplantation. The mRNA expression for neurotrophic factors in both allogenic and autologous groups was significantly higher than that in control groups (p < 0.05). Even though autologous MSC transplantation showed more beneficial effect than that of allogenic MSC transplantation, transplantation of allogenic MSCs also improved functional recovery following SCI.
This study demonstrates that both autologous and allogenic MSC transplantation could be clinically useful therapeutic approaches for treating SCI.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기