한빛사 논문
Sujin Hyung1,2,*, Seung-Ryeol Lee1,*, Jiho Kim1, Youngtaek Kim1, Suryong Kim1, Hong Nam Kim3 and Noo Li Jeon1,4,†
1Department of Mechanical Engineering, Seoul National University, Seoul, Republic of Korea.
2Bio-MAX Institute, Seoul National University, Seoul, Republic of Korea.
3Center for BioMicrosystems, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
4Institute of Advanced Machinery and Design Seoul National University, Seoul, Republic of Korea.
†Corresponding author.
*These authors contributed equally to this work.
Abstract
Demyelinating diseases involve loss of myelin sheaths and eventually lead to neurological problems. Unfortunately, the precise mechanisms remain unknown, and there are no effective therapies. To overcome these limitations, a reliable and physiologically relevant in vitro model is required. Here, we present a three-dimensional peripheral nervous system (PNS) microfluidic platform that recapitulates the full spectrum of myelination, demyelination, and remyelination using primary Schwann cells (SCs) and motor neurons (MNs). The platform enables reproducible hydrogel patterning and long-term stable coculture of MNs and SCs over 40 days in vitro based on three distinct design factors. Furthermore, the on-demand detachable substrate allows in-depth biological analysis. We demonstrated the possibility of mimicking segmental demyelination by lysophosphatidylcholine, and recovery of myelin structure by application of two drugs: benzatropine or methylcobalamin. This 3D PNS disease–on–a–chip may serve as a potential platform for understanding the pathophysiology of demyelination and screening drugs for remyelination.
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