한빛사 논문
Northwestern University, International Institute for Nanotechnology
Sunhong Mina, Min Jun Koa, Hee Joon Jungb,c,d, Wonsik Kime, Seong-Beom Hanl, Yuri Kima, Gunhyu Baea, Sungkyu Leea, Ramar Thangama, Hyojun Choia, Na Lif, Jeong Eun Shina, Yoo Sang Jeona, Hyeon Su Parka, Yu Jin Kima, Uday Kumar Sukumarg, Jae-Jun Songf, Seung-Keun Parkh, Seung-Ho Yui, Yun Chan Kanga, Ki-Bum Leej, Qiang Weik, Dong-Hwee Kiml, Seung Min Hane, Ramasamy Paulmurugangm, Young Keun Kima,n,* and Heemin Kanga,n,*
aDepartment of Materials Science and Engineering, Korea University, Seoul 02841, Republic of Korea
bDepartment of Materials Science and Engineering, Northwestern University, Evanston, IL 60208, USA
cInternational Institute for Nanotechnology, Evanston, IL, USA
dNUANCE Center, Northwestern University, Evanston, IL, USA
eDepartment of Materials Science and Engineering, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea
fDepartment of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul 08308, Republic of Korea
gDepartment of Radiology, Molecular Imaging Program at Stanford, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA
hDepartment of Chemical Engineering, Kongju National University, Cheonan 31080, Republic of Korea
iDepartment of Chemical and Biological Engineering, Korea University, Seoul 02841, Republic of Korea
jDepartment of Chemistry and Chemical Biology, Rutgers University, Piscataway, NJ 08854, USA
kCollege of Polymer Science and Engineering, State Key Laboratory of Polymer Materials and Engineering, Sichuan University, Chengdu 610065, China
lKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea
mDepartment of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Stanford University, Palo Alto, CA 94304, USA
nDepartment of Biomicrosystem Technology, Korea University, Seoul 02841, Republic of Korea
S.M., M.J.K., and H.J. contributed equally to this work
*To whom correspondence should be addressed.
Abstract
Native extracellular matrix (ECM) can exhibit cyclic nanoscale stretching and shrinking of ligands to regulate complex cell–material interactions. Designing materials that allow cyclic control of changes in intrinsic ligand‐presenting nanostructures in situ can emulate ECM dynamicity to regulate cellular adhesion. Unprecedented remote control of rapid, cyclic, and mechanical stretching (“ON”) and shrinking (“OFF”) of cell‐adhesive RGD ligand‐presenting magnetic nanocoils on a material surface in five repeated cycles are reported, thereby independently increasing and decreasing ligand pitch in nanocoils, respectively, without modulating ligand‐presenting surface area per nanocoil. It is demonstrated that cyclic switching “ON” (ligand nanostretching) facilitates time‐regulated integrin ligation, focal adhesion, spreading, YAP/TAZ mechanosensing, and differentiation of viable stem cells, both in vitro and in vivo. Fluorescence resonance energy transfer (FRET) imaging reveals magnetic switching “ON” (stretching) and “OFF” (shrinking) of the nanocoils inside animals. Versatile tuning of physical dimensions and elements of nanocoils by regulating electrodeposition conditions is also demonstrated. The study sheds novel insight into designing materials with connected ligand nanostructures that exhibit nanocoil‐specific nano‐spaced declustering, which is ineffective in nanowires, to facilitate cell adhesion. This unprecedented, independent, remote, and cytocompatible control of ligand nanopitch is promising for regulating the mechanosensing‐mediated differentiation of stem cells in vivo.
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