한빛사 논문
Jeongbin Park1, Sangsu Bae1,2,* and Jin-Soo Kim3,4,*
1Department of Chemistry, 2Institute for Materials Design, Hanyang University, Seoul, South Korea, 3Center for Genome Engineering, Institute for Basic Science, Seoul, South Korea and 4Department of Chemistry, Seoul National University, Seoul, South Korea
*To whom correspondence should be addressed.
Abstract
We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.
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