한빛사 논문
Taehoon Sim1, Bongseo Choi1, Soon Woo Kwon1, Kwang-Soo Kim1, Hyunjun Choi1,2, Alexander Ross1, Dong-Hyun Kim1,2,3,4,*
1Department of Radiology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA
2Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA
3Department of Biomedical Engineering, McCormick School of Engineering, Evanston, IL 60208, USA
4Robert H. Lurie Comprehensive Cancer Center, Chicago, IL 60611, USA
*Corresponding Author: Prof. Dong-Hyun Kim
Abstract
Natural killer (NK) cell-based immunotherapy has been considered a promising cell-based cancer treatment strategy with low side effects for early tumors and metastasis. However, the therapeutic efficacy is generally low in established solid tumors. Ex vivo activation of NK cells with exogenous cytokines is often essential but ineffective to generate high doses of functional NK cells for cancer treatment. Image-guided local delivery of NK cells is also suggested for the therapy. However, there is a lack of noninvasive tools for monitoring NK cells. Herein, magnetic nanocomplexes are fabricated with clinically available materials (hyaluronic acid, protamine, and ferumoxytol; HAPF) for labeling NK cells. The prepared HAPF–nanocomplexes effectively attach to the NK cells (HAPF-NK). An exogenous magnetic field application effectively achieves magneto-activation of NK cells, promoting the generation and secretion of lytic granules of NK cells. The magneto-activated HAPF-NK cells also allow an MR image-guided NK cell therapy to treat hepatocellular carcinoma (HCC) solid tumors via transcatheter intra-arterial infusion. Suppressed tumor growth after the treatment of IA infused magneto-activated NK cells demonstrated a potential enhanced therapeutic efficacy of image guided local delivery of magneto-activated HAPF-NK cells. Given the potential challenges of NK cell cancer immunotherapy against established solid tumors, the effective NK cell labeling with HAPF, magneto-activation, and MRI contrast effect of NK cells will be beneficial to enhance the NK cell-therapeutic efficacy in various cancers.
KEYWORDS : natural killer cell, magneto-activation, nanocomplexes, cell labeling, cancer therapy
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