한빛사 논문
Seung-Hoon Uma,b, Jaehong Leea, In-Seok Songc, Myoung-Ryul Oka, Yu-Chan Kima,d, Hyung-Seop Hana,*, Sang-Hoon Rheeb,*, Hojeong Jeona,d,e,*
aCenter for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology (KIST), Seoul, 02792, Republic of Korea
bDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea
cDepartment of Oral and Maxillofacial Surgery, Korea University Anam Hospital, Seoul, 02841, Republic of Korea
dDivision of Bio-Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul, 02792, Republic of Korea
eKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul, 02841, Republic of Korea
*Corresponding author
Abstract
Hydroxyapatite, an essential mineral in human bones composed mainly of calcium and phosphorus, is widely used to coat bone graft and implant surfaces for enhanced biocompatibility and bone formation. For a strong implant–bone bond, the bone-forming cells must not only adhere to the implant surface but also move to the surface requiring bone formation. However, strong adhesion tends to inhibit cell migration on the surface of hydroxyapatite. Herein, a cell migration highway pattern that can promote cell migration was prepared using a nanosecond laser on hydroxyapatite coating. The developed surface promoted bone-forming cell movement compared with the unpatterned hydroxyapatite surface, and the cell adhesion and movement speed could be controlled by adjusting the pattern width. Live-cell microscopy, cell tracking, and serum protein analysis revealed the fundamental principle of this phenomenon. These findings are applicable to hydroxyapatite-coated biomaterials and can be implemented easily by laser patterning without complicated processes. The cell migration highway can promote and control cell movement while maintaining the existing advantages of hydroxyapatite coatings. Furthermore, it can be applied to the surface treatment of not only implant materials directly bonded to bone but also various implanted biomaterials implanted that require cell movement control.
Keywords : Nanosecond laser, Laser engraving, Hydroxyapatite patterning, Cell migration control, Cell tracking
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