한빛사 논문
Massachusetts General Hospital / Harvard Medical School, Massachusetts General Hospital Research Institute
Hojeong Yu1,2,3, Hoyeon Lee4, Jiyong Cheong3,5, Sang Won Woo6, Juhyun Oh1,2, Hyun-Kyung Oh4, Jae-Hyun Lee3,5, Hui Zheng7, Cesar M. Castro1,8, Yeong-Eun Yoo6, Min-Gon Kim4, Jinwoo Cheon3,5,9, Ralph Weissleder1,2,10, Hakho Lee1,2,*
1Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA 02114, USA. 2Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA. 3Center for Nanomedicine, Insti-tute for Basic Science (IBS), Seoul 03722, South Korea. 4Department of Chemistry, School of Physics and Chemistry, Gwangju Institute of Science and Technology, Gwangju 61005, South Korea. 5Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute, Yonsei University, Seoul 03722, South Korea. 6Department of Nano Manufacturing Technology, Korea Institute of Machinery and Materials, Daejeon 34103, South Korea. 7Biostatistics Center, Massachusetts General Hospital, Boston, MA 02114, USA. 8Department of Medicine, Massachusetts General Hospital, Boston, MA 02114, USA. 9Department of Chemistry, Yonsei University, Seoul 03722, South Korea. 10Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
*Corresponding author.
Abstract
Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography–mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH (express probe for on-site cannabis inhalation). EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 min with a detection limit of 0.17 ng/ml THC, which is below the regulatory guideline (1 ng/ml). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (n = 43) and controls (n = 43). EPOCH detected oral THC in all specimens from cannabis smokers (median concentration, 478 ng/ml) and THC-infused food consumers. Longitudinal monitoring showed a fast drop in THC concentrations within the first 6 hours of cannabis smoking (half-life, 1.4 hours).
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