한빛사 논문
제주대학교
J.C. Harasgamaa,b,1, T.D.W. Kasthuriarachchia,b,1, D.M.K.P. Sirisenaa,b, Hyukjae Kwona,b, Seongdo Leec, Qiang Wana,b,*, Jehee Leea,b,*
aDepartment of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea
bMarine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea
cGeneral Affairs Division, National Fishery Products Quality Management Service, Busan, 49111, Republic of Korea
1These authors contributed equally to this work.
*Corresponding author
Abstract
Interferon regulatory factor 4 (IRF4) is a crucial member of IRF family, which acts as an imperative transcription factor in the development and maturation of multiple lineages of blood cells and also plays a pivotal role in host defense against microbial infections. In the present study, we aimed to investigate the detailed structural and functional aspects of a redlip mullet IRF4 homolog (LhIRF4). The LhIRF4 open reading frame consists of 1347 base pairs encoding 449 amino acids, with the DNA-binding domain sharing significant homology with that of other vertebrate IRF4 homologs. The highest transcription levels of LhIRF4 were observed in the mullet intestine and spleen under normal physiological conditions. Furthermore, a time-dependent upregulation of LhIRF4 was observed in the spleen and head kidney tissues upon pathogenic challenges. When overexpressed in mullet cells, LhIRF4 was localized to the nucleus and significantly stimulated the transcription of several host antiviral genes. Moreover, the overexpression of LhIRF4 strongly inhibited the replication of viral hemorrhagic septicemia virus (VHSV) in vitro. The function of LhIRF4 in regulation of macrophage M2 polarization has also been evidently demonstrated in RAW 264.7 cells. Taken together, our findings indicate the profound role of LhIRF4 in modulating immune responses against microbial infections in redlip mullet.
Keywords : Interferon regulatory factor 4 (IRF4), Redlip mullet, Microbial infection, Antiviral, Macrophage polarization
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