한빛사 논문
Yumeng Xue, Han-Jun Kim, Junmin Lee, Yaowen Liu, Tyler Hoffman, Yi Chen, Xingwu Zhou, Wujin Sun, Shiming Zhang, Hyun-Jong Cho, JiYong Lee, Heemin Kang, WonHyoung Ryu, Chang-Moon Lee, Samad Ahadian, Mehmet R. Dokmeci, Bo Lei, KangJu Lee,* and Ali Khademhosseini*
Y. Xue
State Key Laboratory of Solidification Processing Center for Nano Energy Materials School of Materials Science and Engineering Northwestern Polytechnical University Xi’an 710072, China
Y. Xue, H.-J. Kim, J. Lee, Y. Liu, T. Hoffman, Y. Chen, X. Zhou, W. Sun, S. Zhang, S. Ahadian, M. R. Dokmeci, K. Lee, A. Khademhosseini
Department of Bioengineering and Center for Minimally Invasive Therapeutics (C-MIT) University of California Los Angeles, CA 90095, USA
Y. Xue, B. Lei
Frontier Institute of Science and Technology Xi’an Jiaotong University Xi’an 710054, China
H.-J. Kim, J. Lee, W. Sun, S. Zhang, S. Ahadian, M. R. Dokmeci, K. Lee, A. Khademhosseini
Terasaki Institute for Biomedical Innovation Los Angeles, CA 90064, USA
Y. Liu
College of Food Science Sichuan Agricultural University Yaan 625014, China
H.-J. Cho
College of Pharmacy Kangwon National University Chuncheon 24341, Republic of Korea
J. Lee, W. Ryu
Department of Mechanical Engineering Yonsei University Seoul 03722, Republic of Korea
H. Kang
Department of Materials Science and Engineering Korea University Seoul 02841, Republic of Korea
C.-M. Lee, K. Lee
School of Healthcare and Biomedical Engineering Chonnam National University Yeosu 59626, Republic of Korea
Y.X. and H.-J.K. contributed equally to this work.
*Corresponding author.
Abstract
Silk fibroin (SF) is a promising biomaterial for tendon repair, but its relatively rigid mechanical properties and low cell affinity have limited its application in regenerative medicine. Meanwhile, gelatin-based polymers have advantages in cell attachment and tissue remodeling but have insufficient mechanical strength to regenerate tough tissue such as tendons. Taking these aspects into account, in this study, gelatin methacryloyl (GelMA) is combined with SF to create a mechanically strong and bioactive nanofibrous scaffold (SG). The mechanical properties of SG nanofibers can be flexibly modulated by varying the ratio of SF and GelMA. Compared to SF nanofibers, mesenchymal stem cells (MSCs) seeded on SG fibers with optimal composition (SG7) exhibit enhanced growth, proliferation, vascular endothelial growth factor production, and tenogenic gene expression behavior. Conditioned media from MSCs cultured on SG7 scaffolds can greatly promote the migration and proliferation of tenocytes. Histological analysis and tenogenesis-related immunofluorescence staining indicate SG7 scaffolds demonstrate enhanced in vivo tendon tissue regeneration compared to other groups. Therefore, rational combinations of SF and GelMA hybrid nanofibers may help to improve therapeutic outcomes and address the challenges of tissue-engineered scaffolds for tendon regeneration.
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