한빛사 논문
Intan Rosalina Suhito, Jin Won Kim, Kyeong-Mo Koo, Sun Ah Nam, Yong Kyun Kim,* and Tae-Hyung Kim*
I. R. Suhito, K.-M. Koo, T.-H. Kim
School of Integrative Engineering Chung-Ang University Seoul 06974, Republic of Korea
J. W. Kim, S. A. Nam, Y. K. Kim
Cell Death Disease Research Center College of Medicine The Catholic University of Korea Seoul Korea
J. W. Kim, S. A. Nam, Y. K. Kim
Department of Internal Medicine College of Medicine The Catholic University of Korea St. Vincent’s Hospital, Suwon Republic of Korea
I.R.S. and J.W.K. contributed equally to this work.
*Corresponding author.
Abstract
Organoids that mimic the structural and cellular characteristics of kidneys in vitro have recently emerged as a promising source for biomedical research. However, uncontrollable cellular heterogeneity after differentiation often results in the generation of off-target cells, one of the most challenging issues in organoid research. This study proposes a new method that enables the real-time assessment of kidney organoids derived from stem cells. When placed on a conductive surface, these organoids generate unique electrochemical signals at ≈0.3 V with intensities proportional to the amount of kidney-specific cell types. Off-target cells (i.e., non-kidney cells) produce an electrical signature at 0 V that is distinguishable from other surrounding cell types, enabling non-destructive assessment of both the differentiation, and maturation levels of kidney organoids. The developed platform can be applied to other types of organoids and is thus highly promising as a tool for organoid-based drug screening, toxicity assessment, and therapeutics.
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