한빛사 논문
Dheeraj S. Roy1,2,9,*, Young-Gyun Park3,4,9, Minyoung E. Kim3,9, Ying Zhang5,9, Sachie K. Ogawa1,6,9, Nicholas DiNapoli3, Xinyi Gu3, Jae H. Cho3, Heejin Choi3, Lee Kamentsky3, Jared Martin1, Olivia Mosto5, Tomomi Aida5, Kwanghun Chung2,3,7,* & Susumu Tonegawa1,8,*
1RIKEN-MIT Laboratory for Neural Circuit Genetics at the Picower Institute for Learning and Memory, Department of Biology and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 2Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA. 3Institute for Medical Engineering and Science, Picower Institute for Learning and Memory, Department of Chemical Engineering and Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 4Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea. 5McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 6Department of Psychiatry, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. 7Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea. 8Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 9These authors contributed equally: Dheeraj S. Roy, Young-Gyun Park, Minyoung E. Kim, Ying Zhang, Sachie K. Ogawa.
*Corresponding author.
Abstract
Neuronal ensembles that hold specific memory (memory engrams) have been identified in the hippocampus, amygdala, or cortex. However, it has been hypothesized that engrams of a specific memory are distributed among multiple brain regions that are functionally connected, referred to as a unified engram complex. Here, we report a partial map of the engram complex for contextual fear conditioning memory by characterizing encoding activated neuronal ensembles in 247 regions using tissue phenotyping in mice. The mapping was aided by an engram index, which identified 117 cFos+ brain regions holding engrams with high probability, and brain-wide reactivation of these neuronal ensembles by recall. Optogenetic manipulation experiments revealed engram ensembles, many of which were functionally connected to hippocampal or amygdala engrams. Simultaneous chemogenetic reactivation of multiple engram ensembles conferred a greater level of memory recall than reactivation of a single engram ensemble, reflecting the natural memory recall process. Overall, our study supports the unified engram complex hypothesis for memory storage.
논문정보
관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기