한빛사 논문
Eun Young Jeon, Seung-Hoon Um, Jaeho Park, Youngmee Jung, Cheol-Hong Cheon, Hojeong Jeon,* and Justin J. Chung*
E. Y. Jeon, S.-H. Um, J. Park, Y. Jung, H. Jeon
Biomaterials Research Center Biomedical Research Division Korea Institute of Science and Technology Seoul 02792, Republic of Korea
J. Park
Department of Materials Science and Engineering Seoul National University Seoul 08826, Republic of Korea
Y. Jung
School of Electrical and Electronic Engineering Yonsei University Seoul 03722, Republic of Korea
Y. Jung
Yonsei-KIST Convergence Research Institute Seoul 03722, Republic of Korea
C.-H. Cheon
Department of Chemistry Korea University Seoul 02841, Republic of Korea
H. Jeon
KU-KIST Graduate School of Converging Science and Technology Korea University Seoul 02841, Republic of Korea
J. J. Chung
Transdisciplinary Department of Medicine and Advanced Technology Seoul National University Hospital Seoul 03080, Republic of Korea
J. J. Chung
Department of Medicine Seoul National University College of Medicine Seoul 03080, Republic of Korea
E.Y.J. and S.U. contributed equally to this work.
*Corresponding author.
Abstract
Prompt and robust bone regeneration has been clinically achieved using supraphysiological doses of bone morphogenetic protein-2 (BMP-2) to overcome the short half-life and rapid clearance. However, uncontrolled burst release of exogenous BMP-2 causes severe complications such as heterotopic ossification and soft tissue inflammation. Therefore, numerous researches have focused on developing a new BMP-2 delivery system for a sustained release profile by immobilizing BMP-2 in various polymeric vehicles. Herein, to avoid denaturation of BMP-2 and enhance therapeutic action via localized delivery, a complex coacervate consisting of fucoidan, a marine-derived glycosaminoglycan, and poly-l-lysine (PLL) is fabricated. Superior BMP-2 binding ability and electrostatic interaction-driven engulfment enable facile and highly efficient microencapsulation of BMP-2. The microencapsulation ability of the coacervate significantly improves BMP-2 bioactivity and provides protection against antagonist and proteolysis, while allowing prolonged release. Moreover, BMP-2 containing coacervate is coated on conventional collagen sponges. The bioactivity and localized bone regenerating ability are confirmed through in vitro (human-derived stem cells), and in vivo (calvarial bone defect model) evaluations.
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