한빛사 논문
Huijung Kim # 1, Kübra Solak # 2, Yoojoong Han 1, Yeon-Woo Cho 1, Kyeong-Mo Koo 1, Chang-Dae Kim 1, Zhengtang Luo 3, Hyungbin Son 1, Hyung-Ryong Kim 4*, Ahmet Mavi 5 6*, Tae-Hyung Kim 1*
1School of Integrative Engineering, Chung-Ang University, 84 Heukseuk-ro, Dongjak-gu, Seoul, 06974 Republic of Korea.
2Department of Nanoscience and Nanoengineering, Graduate School of Natural and Applied Sciences, Atatürk University, Erzurum, 25240 Turkey.
3Department of Chemical and Biological Engineering, Hong Kong University of Science and Technology, Kowloon, Hong Kong, 999077 China.
4Department of Pharmacology, College of Dentistry, Jeonbuk National University, Jeonju, 54896 Republic of Korea.
5Department of Nanoscience and Nanoengineering, Institute of Science, Atatürk University, Erzurum, 25240 Turkey.
6Department of Mathematics and Science Education, Education Faculty of Kazim Karabekir, Atatürk University, Erzurum, 25240 Turkey.
#Contributed equally.
*Corresponding author.
Abstract
Direct messenger ribonucleic acid (mRNA) delivery to target cells or tissues has revolutionized the field of biotechnology. However, the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers. Herein, we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) via electrically controlled mRNA delivery. To find optimal electrical conductivity and mRNA-loading capacity, the polypyrrole-graphene oxide (PPy-GO) hybrid film was electropolymerized on indium tin oxide substrates. We found that the fluorescein sodium salt, a molecule partially mimicking the physical and chemical properties of mRNAs, can be effectively absorbed and released by electrical stimulation (ES). The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation. This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.
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