한빛사 논문
Jung Hee Wee1†, Ki‑Dong Yoo2†, Sung Bo Sim3†, Hyun Joo Kim4†, Han Joon Kim5, Kyu Nam Park5, Gee‑Hee Kim2, Mi Hyoung Moon6, Su Jung You4, Mi Yeon Ha4, Dae Hyeok Yang4, Heung Jae Chun4,7,8*, Jae Hoon Ko9 and Chun Ho Kim10
1Department of Emergency Medicine, Yeouido St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 07345, Republic of Korea.
2Division of Cardiology, Department of Internal Medicine, St. Vincent’s Hospital, Suwon 16247, Republic of Korea.
3Department of Thoracic and Cardiovascular Surgery, Bucheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Bucheon, 14647 Seoul, Republic of Korea.
4Institute of Cell and Tissue Engineering, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
5Department of Emergency Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
6Department of Thoracic and Cardiovascular Surgery, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
7Department of Medical Life Sciences, The Catholic University of Korea, Seoul 06591, Republic of Korea.
8Department of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
9Smart Textiles R&D group, Korea Institute of Industrial Technology (KITEC), Ansan 31056, Republic of Korea.
10Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, 01812 Seoul, Republic of Korea.
†Jung Hee Wee, Ki-Dong Yoo, Sung Bo Sim and Hyun Joo Kim contributed equally to this work.
*Corresponding author : Correspondence to Heung Jae Chun.
Abstract
Background: Although the use of cardiac patches is still controversial, cardiac patch has the significance in the field of the tissue engineered cardiac regeneration because it overcomes several shortcomings of intra-myocardial injection by providing a template for cells to form a cohesive sheet. So far, fibrous scaffolds fabricated using electrospinning technique have been increasingly explored for preparation of cardiac patches. One of the problems with the use of electrospinning is that nanofibrous structures hardly allow the infiltration of cells for development of 3D tissue construct. In this respect, we have prepared novel bi-modal electrospun scaffolds as a feasible strategy to address the challenges in cardiac tissue engineering .
Methods: Nano/micro bimodal composite fibrous patch composed of collagen and poly (D, L-lactic-co-glycolic acid) (Col/PLGA) was fabricated using an independent nozzle control multi-electrospinning apparatus, and its feasibility as the stem cell laden cardiac patch was systemically investigated.
Results: Nano/micro bimodal distributions of Col/PLGA patches without beaded fibers were obtained in the range of the 4-6% collagen concentration. The poor mechanical properties of collagen and the hydrophobic property of PLGA were improved by co-electrospinning. In vitro experiments using bone marrow-derived mesenchymal stem cells (BMSCs) revealed that Col/PLGA showed improved cyto-compatibility and proliferation capacity compared to PLGA, and their extent increased with increase in collagen content. The results of tracing nanoparticle-labeled as well as GFP transfected BMSCs strongly support that Col/PLGA possesses the long-term stem cells retention capability, thereby allowing stem cells to directly function as myocardial and vascular endothelial cells or to secrete the recovery factors, which in turn leads to improved heart function proved by histological and echocardiographic findings.
Conclusion: Col/PLGA bimodal cardiac patch could significantly attenuate cardiac remodeling and fully recover the cardiac function, as a consequence of their potent long term stem cell engraftment capability.
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