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[101Bio] Cell Immortalization Kits!
모아바이오
Product Name: SV40 T Antigen Cell Immortalization Kit
Description: It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, socalled replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. Simian virus 40 (SV40) T antigen has been shown to be the simplest and most reliable agent for the immortalization of many different cell types. Recent studies have also shown that SV40 T antigen can induce Telomerase activity in the infected cells.
Size: 5 x 20 µl Shipping: Dry ice Store at -80 °C (stable up to 6 months)
Product Name: hTERT Cell Immortalization Kit
Description: It is well known that primary cells only undergo a pre-determined and finite number of cell divisions in culture. After limited population doublings (the number of which varies by species, cell type, and culture conditions), primary cells enter a state, so-called replicative senescence, where they can no longer divide. To surpass senescence, several methods exist for immortalizing mammalian cells in culture. The most recently discovered approach to cell immortalization is through the expression of Telomerase Reverse Transcriptase protein (TERT). It is particularly useful for cells that are most affected by telomere length, including many human cell types. This protein is usually silenced in most somatic cells. These cells can avoid replicative senescence by maintaining sufficient telomere lengths when hTERT is exogenously introduced. However, over-expression of hTERT in some cell types (especially in epithelial cells) fails to induce cell immortalization.
Size: 5 x 20 µl Ship: Dry ice Store at -80 °C (stable up to 6 months)
Virus Transduction Enhancer
Description: This reagent can increase the transduction rate of lentivirus or retrovirus up to 10-fold in most cells. It is a mixture of polymers optimized for the infection of lentivirus or retrovirus to most cells. is provided as a 500x solution, sufficient for 100 transductions in a 24 well plate. It is intended for retroviral or lentiviral transduction.
Size: 100 µl (PV100 ) or 500 µl ( PV500 ) Ship: Ambient temperature Storage: This product is stable for 3 months when stored at 4 °C or 6 months at -20 °C.
Virus Transduction Enhancer
Description: This reagent can increase the transduction rate of lentivirus or retrovirus up to 10-fold in most cells. It is a mixture of polymers optimized for the infection of lentivirus or retrovirus to most cells. is provided as a 500x solution, sufficient for 100 transductions in a 24 well plate. It is intended for retroviral or lentiviral transduction.
Size: 100 µl (PV100 ) or 500 µl ( PV500 ) Ship: Ambient temperature Storage: This product is stable for 3 months when stored at 4 °C or 6 months at -20 °C.
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