한빛사 논문
서울대학교
Haneul Yooa,1, Junghyun Shina,1, Jieun Simc,d,e, Hyunmin Choc,d, Seunghun Hongb,*
aDepartment of Physics and Astronomy, Seoul National University, Seoul, 08826, Republic of Korea
bDepartment of Physics and Astronomy, And Institute of Applied Physics, Seoul National University, Seoul, 08826, Republic of Korea
cDepartment of Nanobiotechnology, KRIBB School of Biotechnology, University of Science and Technology, Daejeon, 34113, Republic of Korea
dBionano Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
eBioNano Health Guard Research Center (H-GUARD), Daejeon, 34141, Republic of Korea
1These authors contributed equally to this work.
*Corresponding author
Abstract
We developed a reusable magnetic surface plasmon resonance (SPR) sensor chip for detecting various target molecules repeatedly in a conventional SPR system. Here, ferromagnetic patterns on a SPR sensor chip were utilized to trap a layer of magnetic particles, and they were utilized as a solid substrate for SPR sensing in a conventional SPR system. After a sensing experiment, the used magnetic particles were removed by external magnetic fields, and a new layer of magnetic particles was immobilized to the SPR sensor chip for additional sensing measurements. Since magnetic particles were trapped on the ferromagnetic patterns, we could use our reusable SPR chip for SPR sensing measurements in a traditional SPR system without any applied magnetic fields. Significantly, ferromagnetic patterns on the sensor chip surface deflected the strong external fields, so that the large aggregation of magnetic particles on the sensor surface was reduced. We demonstrated using a single reusable SPR sensor chip to measure the nucleoprotein (NP) of H1N1 influenza virus solution ranging repeatedly for more than 7 times without significant signal degradation. Also, different target molecules could be repeatedly measured in a single SPR chip. Since our reusable SPR sensor chip can be repeatedly used in a conventional SPR system without any chemical processes for refreshment, the cost for SPR sensing should be significantly reduced. In this case, our reusable SPR sensor chip can be a major breakthrough and can be used for versatile practical applications of SPR sensors.
Keywords : Surface plasmon resonance; Biosensor; Reusable; Ferromagnetic pattern; Magnetic trapping-releasing; Influenza
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