한빛사 논문
University of Pennsylvania, Corporal Michael J. Crescenz VA Medical Center
Su-Jin Heo1,2,3, Kwang Hoon Song3,4,*, Shreyasi Thakur5,*, Liane M. Miller1,2,*, Xuan Cao3,6, Ana P. Peredo1,2, Breanna N. Seiber1,2, Feini Qu1,2, Tristan P. Driscoll1,2, Vivek B. Shenoy3,4,6, Melike Lakadamyali3,5, Jason A. Burdick2,3,4 and Robert L. Mauck1,2,3,4,†
1McKay Orthopaedic Research Laboratory, Department of Orthopaedic Surgery, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
2Translational Musculoskeletal Research Center, Corporal Michael J. Crescenz VA Medical Center, Philadelphia, PA, USA.
3Center for Engineering Mechanobiology, University of Pennsylvania, Philadelphia, PA USA.
4Department of Bioengineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA.
5Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
6Department of Materials Science Engineering, School of Engineering and Applied Science, University of Pennsylvania, Philadelphia, PA, USA.
†Corresponding author.
*These authors contributed equally to this work.
Abstract
Dense matrices impede interstitial cell migration and subsequent repair. We hypothesized that nuclear stiffness is a limiting factor in migration and posited that repair could be expedited by transiently decreasing nuclear stiffness. To test this, we interrogated the interstitial migratory capacity of adult meniscal cells through dense fibrous networks and adult tissue before and after nuclear softening via the application of a histone deacetylase inhibitor, Trichostatin A (TSA) or knockdown of the filamentous nuclear protein Lamin A/C. Our results show that transient softening of the nucleus improves migration through microporous membranes, electrospun fibrous matrices, and tissue sections and that nuclear properties and cell function recover after treatment. We also showed that biomaterial delivery of TSA promoted in vivo cellularization of scaffolds by endogenous cells. By addressing the inherent limitations to repair imposed by nuclear stiffness, this work defines a new strategy to promote the repair of damaged dense connective tissues.
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